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PhyTip® 5K Gel Filtration Column for Automated High Throughput Desalting and Buffer Exchange of Functional Proteins
1 Mar 2013In this application note it is demonstrated that the PhyTip 5K gel filtration columns and the MEA Personal Purification System represent a high throughput solution for rapid desalting and buffer exchange. PhyTip 5K gel filtration columns can process 12 samples in 30 minutes, which is significantly faster compared to the laborious and time consuming dialysis procedure.
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PhyTip® Columns and MiniPrep PhyTip® Columns
BiotagePhyTip® columns use dual-flow chromatography within pipette tips to automate purification workflows. This technology delivers high reproducibility and exceptional sample purity for efficient, reliable results.
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Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.