PhyNexus PhyTip® Columns for Rapid Micro-Volume Scale Capture, Purification and Enrichment of Proteins and Antibodies

11 Jun 2013

The introduction of recombinant proteins that poses amino acid-tagged sites has resulted in a mechanism for many researchers to specifically extract proteins of interest from a mass of unwanted macromolecules in order to study the protein’s structure and function. However, the isolation of recombinant proteins and antibodies for analysis by any of today’s high sensitivity detection techniques such as mass spectrometry or protein biochips involves a tedious and time consuming macro-scale process. In addition these same isolation processes result in an end product that may not retain functional integrity and therefore the end result could be compromised. In this application note read how PhyNexus, Inc. has developed a unique system to overcome the issue of producing sufficient quantities of purified and fully functional protein at a micro-volume scale.

PhyTip® Columns and MiniPrep PhyTip® Columns

Biotage

PhyTip® columns use dual-flow chromatography within pipette tips to automate purification workflows. This technology delivers high reproducibility and exceptional sample purity for efficient, reliable results.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody PurificationProtein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Antibody ProductionAntibody production is essential for a wide range of applications in biochemistry and immunology. Ensuring high-quality monoclonal and polyclonal antibodies are produced is key. Explore antibody production tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.