ResourceSeparations
Phosphoproteomics of Cell Lysates Using Titatium Dioxide Phosphopeptide Enrichment Combined With LC-MS/MS
10 Oct 2012Cellular processes, such as signal transduction, cell division, metabolism, apoptosis, cell motility, and carcinogenesis, are controlled through reversible phosphorylation of proteins. Abnormally phosphorylated proteins have been associated with the pathology of cancer, diabetes, and Alzheimer’s disease. Phosphorylated amino acids exist at a low natural stoichiometry, and require selective enrichment strategies for the concentration of phosphorylated peptides and proteins prior to analysis, usually by mass spectrometry. This case study, from Protea, highlights the use of titanium dioxide (TiO2) mediated phosphopeptide enrichment for the characterization of phosphoproteins in cell lysates.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein EnrichmentPhosphorylation