ResourceLife Sciences
Phenotypic Assessment of Toxicity using a Human Hepatocyte Co-Culture Model
4 Aug 2015Development of predictive in-vitro assays for early toxicity evaluation is extremely important for improving the drug development process and reducing drug attrition rates during clinical trials. This application note presents an alternative model in which the cells remain functional over a prolonged treatment period, by developing a phenotypic assay which assesses multi-parameter readouts of general and mechanism-specific hepatotoxicity. The hepatocyte islands (~500 mm diameter) were imaged with an ImageXpress® Micro XLS high-content imaging system. Automated analysis was performed using an optimized Custom Module in MetaXpress® software.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.In Vivo Imaging Systems<i>In vivo</i> imaging systems, including pre-clinical imaging systems and medical imaging systems are used to non-invasively visualize and capture images of live animals and plants. Monitor the natural processes or diseases of your subjects using small-animal pre-clinical imaging systems, including single photon positron emission tomography (SPECT), positron emission tomography (PET), computed tomography (micro-CT), magnetic resonance imaging (MRI), X-ray radiography, ultrasound, fluorescence and bioluminescence imagers. Multimodal systems and software solutions are also available for correlative analysis of organ, tissue, cell, or molecular-level processes. Find the best in vivo imaging products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HepatotoxicityHepatocytesPhenotypic ScreeningPhenotypic screening assesses cellular responses to compounds, enabling drug discovery and target identification. This technique is pivotal in finding effective treatments for complex diseases. Discover phenotypic screening platforms and tools with peer-reviewed comparisons and pricing in our directory.