ResourceSpectroscopy

Performing on-particle protein digestion reduces total processing time and increases unique protein identifications

23 Dec 2019

In this application note, Ceres Nanotechnologies presents a simplified "on-particle" digestion method, which reduces processing time by performing protein digestion and clean up while the proteins are still bound to the Nanotrap® particles, which leads to a larger number being identified via LC-MS.

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. NanoparticlesNanoparticles are between 1-100nm in size. Nanoparticles can be used for a wide variety of applications including biomedical, catalysis and electronics.
Performing on-particle protein digestion reduces total processing time and increases unique protein identifications