Performance characteristics of the Agilent Oligo Pro ll System

In this application note, Agilent provides experimental data relating to the performance characteristics of the Agilent Oligo Pro II System. This automated capillary electrophoresis instrument is designed for high-throughput separation and purity analysis of the single-stranded DNA (ssDNA) and RNA oligonucleotides (oligos). As oligos are synthesized, small amounts of impurities can be introduced at each step. Different synthesis methods and purification efficiencies can impact the quality of the sample, requiring assessment of the oligo to ensure it is of the purity level needed for downstream applications such as PCR, gene assembly, and microarray/hybridization assays.