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Perform a six-color probe-based assay without signal crosstalk and calibration effort

Analytik Jena GmbH+Co. KG
17 Nov 2023

Currently, most commercial detection assays identify up to four different targets, and rarely up to five genes. A major challenge lies in the precise separation of individual fluorescence signals across different channels. This is one of the main reasons why there are still relatively few assays using more than five different dyes for detection.

Thanks to the new technology featuring a unique multicolor LED light source and extended wavelength ranges in qTOWERiris, along with a clever combination of dyes used for probe labeling, real-time PCR with up to six targets, without crosstalk and the need for expensive calibration reagents, is now possible.

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qTOWER iris: The Real-Time PCR-Thermocycler

Analytik Jena GmbH+Co. KG

No matter where your journey takes you in the world of qPCR, this real-time PCR thermal cycler is ready for anything, giving you full control and freedom of choice - from consumables to assays to colour modules. The instrument is operating quietly, smoothly and delivers reliable results on six channels. The temperature and readout accuracy are unsurpassed. With this open system you are well equipped for all your projects.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.Calibration

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Perform a six-color probe-based assay without signal crosstalk and calibration effort