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Peptide Separations Using Reverse Phase RediSep® Columns

Peptide Separations Using Reverse Phase RediSep® Columns

2 Jul 2015

This application note describes a generalized approach to separating peptide mixtures into fractions on reusable reversed phase RediSep® C18 columns from Teledyne Isco. Peptide mixtures may be separated into individual peptide species. If the separate and more specific peptides can be contained in a single fraction, this can greatly expedite many scientific processes.

CombiFlash® Torrent

Teledyne Labs

Get the ease of use and reliability provided by CombiFlash and RediSep for Development Scale purification with the CombiFlash Torrent Purification System from ISCO. One liter per minute flow rates rapidly purify up to 300 grams of sample. The 100 psi pumps accurately deliver a reproducible binary gradient. The CombiFlash Torrent system is designed to be flexible for a variety of large-scale purification needs, with various methods of sample introduction and collection.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Amino AcidsPeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.Reversed-Phase LC
Peptide Separations Using Reverse Phase RediSep® Columns