SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Peptide-Antibody Interaction Specificity Using SPRi

HORIBA Scientific
3 May 2016

This application note demonstrates the relevance of using SPRi technology for research into peptide-antibody interactions. First, streptavidin grafting is performed by using GenOptics pyrrole electro-polymerization protocol. A mix of pyrrole and pyrrolated streptavidin is co-polymerized on the gold layer of a glass prism. Biotinylated peptides are then injected in the detection cell and create a homogenous peptide layer, due to the strong affinity between streptavidin and biotin. Use of SPRi technology allows real-time studies of peptide-antibody interactions on a large number of spots analyzed in parallel.

Related products

Request Quote for All Products

OpenPlex - Flexible Surface Plasmon Resonance Imaging System

HORIBA Scientific

Designed to meet the demands of biologists, biochemists and biophysicists, the OpenPlex is a flexible surface plasmon resonance imaging system. OpenPlex is your companion for the development of label-free and multiplexed bio-assays and molecule detection. It is a robust and compact system designed for simple use and high versatility. Its open format, dedicated sensor chips and manual operation enable numerous types of experiments to be explored without compromise, covering chemistry, biochemistry, physico-chemistry and biomolecular interactions. Open your research areas with OpenPlex! Choose between three different flow cell configurations without concession: • Normal flow cell for standard surface plasmon resonance imaging experiments • Window flow cell, compatible with fluorescence measurements • Cuvette cell, compatible with electrochemistry measurements

(0)

Links

HORIBA ScientificCompany website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.Surface Plasmon ResonancePeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.Secondary Antibodies

Related resources

Peptide-Antibody Interaction Specificity Using SPRi