ResourceLife Sciences
PathHunter™ ß-arrestin Flash Assay with Mithras LB 940
13 Oct 2016This application note describes how the PathHunter™ Flash Detection Kit can detect ß-arrestin recruitment by a GPCR in 30 seconds. The speed of this ß-arrestin chemiluminescent assays, unlike lengthy reporter gene assays, greatly minimize the opportunity for off-target effects. The Mithras LB 940, a multimode plate reader with a unique optical design which ensures optimized performance, is used to detect bioluminescence produced in the assay. Therefore, the PathHunter™ Flash assay provides the ability to read the signal within much shorter time than with the glow-type format.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.ChemiluminescenceAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.