ResourceLife Sciences
Patching Transiently Transfected Ion Channels on an Automated Electrophysiology System
13 Jul 2011To broaden the applicability of automatic patch clamp systems, scientists from Molecular Devices tested assays with cells transiently transfected with ion channel genes. Two chimeric cationic channels, an α7 acetylcholine-glycine chimeric receptor (α7-GlyR) and an α7 acetylcholine-serotonin receptor (α7-5HTR), were transfected into HEK-293 parental cells. After cell harvesting, acetylcholine-induced currents were recorded from both α7-GlyR- and α7-5HTR-transfected cells.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. 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