SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Optimizing Settings to Validate the BMG LABTECH CLARIOstar HTS Microplate Readers with the Transcreener TR-FRET Assays

BellBrook Labs
23 Oct 2014

This application protocol describes the optimal instrument parameters used to validate the CLARIOstar® Microplate Reader with the Transcreener ADP2 TR-FRET (3011) and Transcreener AMP2/GMP2 TR-FRET (3020) assays from Bellbrook Labs. Transcreener® is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes. Many of these enzymes catalyze the covalent regulatory reactions that are central to cell signaling; e.g., phosphorylation, methylation, and are of high interest as therapeutic targets. The Transcreener® TR-FRET Assays are a single step, competitive immunoassay for direct detection of nucleotides with a far red time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. The use of a red tracer minimizes interference from fluorescent compounds and light scattering. The Transcreener® TR-FRET Assays are designed specifically for HTS with a single addition, mix-and-read format.

Related products

Request Quote for All Products

Transcreener® ADP² Kinase Assays

BellBrook Labs

The Transcreener® ADP² Kinase Assay Kit universally detects ADP produced by any kinase or ATPase using a direct mix‑and‑read format, eliminating coupling steps. Available in FP, FI, and TR‑FRET formats with far‑red tracers to minimize interference, it's HTS-ready across 96‑, 384‑, and 1536‑well plates with robust Z' ≥ 0.7 performance.

(3)

Links

BellBrook LabsCompany website

Tags

MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.TR-FRET

Related resources

Optimizing Settings to Validate the BMG LABTECH CLARIOstar HTS Microplate Readers with the Transcreener TR-FRET Assays