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Optimizing DNA extraction from blood using prepGEM™ and forensicGEM™ Blood kits

ZyGEM Corporation Limited
22 Aug 2013

In general, DNA does not need extensive purification for the polymerase chain reaction (PCR). The key to simplifying nucleic acid preparation is to develop procedures that minimize labour and cost, but are sufficiently rigorous to remove inhibitory substances. Blood is notoriously difficult to work with because it contains nu¬merous inhibitors. This application note presents two methods for DNA extraction from blood using the prepGEM™ and forensicGEM™ Blood kits.

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forensicGEM™

ZyGEM Corporation Limited

forensicGEM™ has been formulated for high-throughput DNA extraction from crime samples. The forensicGEM™ kits have been specifically designed to be compatible with high-multiplex STR profiling kits and all reagents are rigorously Quality Controlled to test for efficacy and for the absence of human DNA. ZyGEM technology is ideally suited to the large-scale batch processing of reference samples for forensic analysis. Validated by ESR New Zealand, one of the largest forensics laboratories in the world, forensicGEM™ is a rapid, high throughput processing technology for storage card and buccal swab reference samples. ZyGEM also offers a comprehensive range of optimized kits for the preparation of crime scene and CODIS samples. All forensicGEM™ kits are rigorously tested at a leading forensic laboratory for efficacy and purity. The basis of all of the kits is the forensicGEM™ proteinase, but special formulations have been developed for different substrates forensicGEM™ DNA EXTRACTION HAS MANY ADVANTAGES: Closed–tube (protects sample integrity) Hands–off (saves labor costs) One-step (maximizes yields and so increases the chance of obtaining a usable profile) Easily automated (compatible with most low cost, off-the-shelf liquid handling robots) Validated for forensic analysis

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Blood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.Forensic InvestigationForensic investigation is a diverse collection of scientific techniques and methods for the identification and characterization of chemicals relating to crime scene investigations.

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Optimizing DNA extraction from blood using prepGEM™ and forensicGEM™ Blood kits