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Optimizing Chemiluminescent Western Blots

LICORbio
10 Sept 2018

The Odyssey Fc Imaging System is a CCD-based imager that detects chemiluminescent signal, visible signal at 600 nm, and near-infrared fluorescent signals at 700 nm and 800 nm wavelengths. This technical note highlights both chemiluminescent and near-infrared Western blots using this system. It can detect near-infrared fluorescent markers on a chemiluminescent blot, providing a less expensive alternative to HRP-labeled markers.

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Odyssey XF Imaging System

LICORbio

The Odyssey XF is the perfect entry point for labs transitioning from qualitative chemiluminescent Western blotting to more robust quantitative data analysis. Drawing from more than 30 years of imaging expertise, the Odyssey XF offers unmatched dynamic range with the click of a mouse. In addition to two NIR lasers, it has LED and CCD capabilities, providing multi-functional versatility for greater value.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Optimizing Chemiluminescent Western Blots