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Optimizing analysis and purification of a synthetic peptide using PLRP-S columns
24 May 2023Reversed-phase ion-pair chromatography has become increasingly useful for the analysis and purification of synthetic peptides using mobile phases containing trifluoroacetic acid (TFA) as an ion-pair reagent. This application note from Agilent describes the analysis of a synthetic peptide and how to scale-up methods using an Agilent PLRP-S analytical HPLC column.
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PLRP-S for Biomolecules
Agilent TechnologiesPLRP-S for Biomolecules products are available in a range of pore sizes and particle sizes, all with identical chemistry and fundamental adsorptive characteristics. The particles are inherently hydrophobic so there is no bonded phase, alkyl ligand required for reversed-phase separations.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.