Optimized methodology for sequential skin tissue extraction of RNA and protein from small human biopsies

3 Dec 2019

Skin tissue, although easily accessible, is difficult to process owing to its natural resistance to mechanical shearing and high levels of RNase and proteases. In this application note, MP Biomedicals uses two extraction techniques to disrupt the biopsy samples, homogenization and bead beating.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein Purification
Optimized methodology for sequential skin tissue extraction of RNA and protein from small human biopsies