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Optimization of Mobile Phase Composition for the Analysis of Synthetic Oligonucleotides using a Clarity® Oligo-XT Column
23 Jul 2017Mobile phase composition is important when developing methods for characterization of OGNs. In this application note, we investigate the use of two mobile phase compositions - TEA HFIP and DIEA HFIP - with Clarity Oligo-XT, a novel core-shell material optimized for synthetic oligonucleotide applications.
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Clarity LC BioSolutions for Synthetic DNA/RNA
Phenomenex IncPurification and Analysis of Synthetic OligonucleotidesThe Clarity BioSolutions LC portfolio includes novel RP-LC and IEX-LC columns for purifying and characterizing synthetic DNA / RNA used in biological research, therapeutic development, and biochemical manufacturing.Clarity Oligo-MS™: LC/MS analysisClarity Oligo-RP™: RP-LC purification and analysisClarity Oligo-WAX™: IEX-LC purification
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Chromatography Method DevelopmentChromatography method development is the process of designing and optimizing chromatographic techniques to separate and analyze complex mixtures. This includes selecting appropriate stationary and mobile phases, determining flow rates, and optimizing detection methods. Successful method development is essential for high-quality results in fields like pharmaceuticals, food safety, and environmental analysis. Explore chromatography method development tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.