Optimization of an Agilent NGS automated workflow for the characterization of NSCLC DNA samples in a molecular diagnostic and anatomic pathology laboratory

29 Aug 2023

This white paper from Agilent presents a study in which researchers from the Anatomical Pathology laboratory characterized lung cancer samples using a complete Agilent workflow, reliably detecting variants that are not easily detectable with other systems in a single, DNA-based assay, and increasing reproducibility and lab productivity.

Magnis NGS Prep System

Agilent Technologies

The Magnis system provides a complete workflow for NGS library preparation that is fully automated and delivers reproducible results. Built on proven Agilent SureSelect XT HS2 chemistry, start from total RNA with integrated cDNA conversion or unfragmented DNA with onboard enzymatic fragmentation for increased workflow efficiency.

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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Optimization of an Agilent NGS automated workflow for the characterization of NSCLC DNA samples in a molecular diagnostic and anatomic pathology laboratory