SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

Optimization of a Multi-Mode Detection Model for Measuring Real-Time Cellular Respiration and Mitochondrial Function Using Fluorophoric Biosensors

Agilent Technologies
26 Apr 2015

Characterization of cellular metabolism is being aided by the development of new tools designed to provide ease-of-use, higher throughput, and multiplexed data markers for analysis. This application note demonstrates that Luxcel probes can be detected using a variety of BioTek readers to inform cellular metabolic analysis. The results also show that BioTek readers and Luxcel probes are compatible for analyzing extracellular oxygen consumption rates and intracellular O2 levels of live cell response to drug treatment.

Related products

Request Quote for All Products

Agilent BioTek Synergy H1 Multimode Reader

Agilent Technologies

Agilent BioTek Synergy H1 is a modular multi-mode microplate reader, with monochromator-based optics and filter-based optics. The proprietary Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change.

(21)

Links

Agilent TechnologiesCompany website

Tags

Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :MitochondriaOxygen Testing

Related resources

Optimization of a Multi-Mode Detection Model for Measuring Real-Time Cellular Respiration and Mitochondrial Function Using Fluorophoric Biosensors