One-Step RT-qPCR products for high-throughput research applications

At each cycle during PCR, the quantity of DNA is measured in real time by using a variety of fluorescent chemistries. The most common approaches to generate a fluorescent signal are to use either hydrolysis probes such as TaqMan^® probes, or a double-stranded DNA binding dye such as SYBR^® Green dye. The selection of fluorescent chemistry is dependent upon factors such as the application, cost, and whether the assay is a singleplex or multiplex assay. DNA-binding dyes are preferred for singleplex, low-throughput assays since they are easier to design, have lesser setup time, and are more cost efficient. Fluorescent probes are more commonly employed in high-throughput, multiplex assays that require higher specificity.