ResourceSpectroscopy
One-minute drug panel screening with LC-HRMS
15 Jun 2023In this application note, Phenomenex details a study that was conducted to validate a bioanalytical method for preliminary screening of nine drugs in urine. The study aimed to address gaps in commercially available screening technologies by utilizing high-resolution mass spectrometry (HRMS) as an alternative. The LC-MS method with a Kinetex® 2.6 µm EVO C18 LC column allowed ultra-fast screening (cycle time ~1 min) for analytes that could not be screened using traditional methods. Complete hydrolysis was achieved within 5 minutes using a novel enzyme. The method met specifications for linearity, lower limit of quantitation (LLOQ), precision and accuracy, specificity, selectivity, and matrix effects.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.