ResourceSpectroscopy
Oligonucleotide quantification
31 Aug 2020In this application note, MilliporeSigma illustrates how to quantify oligonucleotides and explains that the concentration of nucleic acids in solution is readily quantified in a spectrophotometer when exposed to ultraviolet (UV) light at 260 nm.
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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.SpectrophotometrySpectrophotometry is a technique used to measure the absorption or transmission of light through a sample. It is commonly used in chemical analysis, environmental monitoring, and quality control. Explore spectrophotometry tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.