ResourceLife Sciences
Oligonucleotide melting temperature
31 Aug 2020In this application note, MilliporeSigma explains the two methods used to determine the melting temperature of an oligonucleotide, the nearest neighbor method and the basic method.
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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Sample HandlingProper and careful handling of samples is important in scientific studies to ensure the integrity of specimens. This will prevent deterioration and cross contamination, and improve the accuracy of sample tracking procedures.