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NucleoCounter® NC-202™: Cell Count & Viability of Yeast Cells using Via2-Cassette™

ChemoMetec
12 Jul 2021

The NucleoCounter® NC-202™ automated cell counter and NC-View™ software perform cell counting and viability analyses on a broad range of eukaryotic cells. The Via2-Cassette™ provides a simple and robust method to determine cell concentration and viability with the NucleoCounter® NC-202™. This application note demonstrates the cell concentration and viability of Saccharomyces and Schizosaccharomyces yeast cells using the Via2-Cassette™ and Reagent Y200 (yeast permeabilization buffer).

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Via2-Cassette™ cell counting device

ChemoMetec

The Via2-Cassette™ is our unique cell sampling device, eliminating human error by combining cell sampling, staining and loading into a single press on a piston. Individually volume-calibrated, it guarantees the highest precision in cell counting. The Via2-Cassette™ is a disposable plastic unit containing the fluorescent dyes used for analysis to ensure user safety and operational ease.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.CytometryCytometry is a broad term for the quantitative analysis of cells and cell systems. The most popular methods to study cytometry are flow cytometry and image cytometry.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.

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NucleoCounter® NC-202™: Cell Count & Viability of Yeast Cells using Via2-Cassette™