ResourceLife Sciences
Novel Tumor Targeting Near Infrared Fluorescent Probes
10 Apr 2011This application note by Caliper Life Sciences evaluates the pharmacokinetic/pharmacodynamic profile of fluorescent probes using optical imaging. HER2-targeting agents are labeled with NIR dyes for fluorescence detection. In vivo imaging shows tumor targeting at 3 hours after intravenous injection in a subcutaneous SKOV3 tumor model. HER2 has become an important prognostic and predictive factor, as well as a target for molecular therapies, in specific types of breast cancer.
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. 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