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Novel Approach to Separating Amphetamine Enantiomers from Urine Using Lux® 3 μm AMP

Phenomenex Inc
25 Feb 2017

This application note describes an alternative method for quantification of amphetamine enantiomers from urine to off-line derivatization followed by GC/MS or LC/MS. The novel approach utilizes a Gemini® C18 trapping column and Lux 3 μm AMP analytical column to carry out the reversed phase separation of amphetamine and substituted amphetamines without the need for derivatization. This article highlights the benefits of this method over the traditional method, which include greater reliability, greater sample throughput and being less time-consuming.

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Lux™ Chiral LC & SFC Columns

Phenomenex Inc

Polysaccharide Chiral HPLC and SFC Columns at a Very Affordable Price. Optimize your chiral separation and chiral purification with Lux® polysaccharide chiral HPLC columns. The Lux family of amylose and cellulose chiral selectors provides a variety of complementary selectivities that allow you to screen for the most effective chiral separation under Reversed Phase, Polar Organic, Normal Phase, and SFC conditions. With numerous publications in peer reviewed journals and countless applications being developed, Lux chiral HPLC columns are rapidly becoming the premier choice for simplified chiral purification and chiral separation worldwide.   Column Details: Phase: Amylose-2, Cellulose-1,2,3,4 Particle Size: 3, 5, 10, 20 µm Pore Size: 1000 Å Length: 50, 100, 150, 250 mm ID: 2.0, 4.6, 10.0, 21.2, 30.0, 50.0 mm

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.Drugs of AbuseA drug of abuse is a drug that has been taken for non-medical purposes. Such drugs can have mind-altering effects, with some causing dependence and addiction.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.

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Novel Approach to Separating Amphetamine Enantiomers from Urine Using Lux® 3 μm AMP