ResourceLife Sciences
NGS: Best practices for sample homogenization
17 Aug 2020The overall success of a next-generation sequencing (NGS) workflow is greatly impacted by the various sample preparation steps. Obtaining adequate quantity and acceptable quality of nucleic acids from the sample is highly dependent on the efficiency of homogenization.
In this application compendium, we present best practices for sample homogenization based on the protocols developed and tested by scientists. Applied across diverse sample types, such as biological tissue, food, and fecal samples, these methods demonstrate how high-quality, high-yield nucleic acids can be obtained by choosing optimized bead-beating homogenization protocols with Bertin’s Precellys homogenizers.
The eBook covers:
- Robust and unbiased microbial RNA extraction workflow
- MicroRNA expression profiling of individual rat hypothalamic nuclei
- Transcriptomic analysis of cheese-ripening microbial communities
- Microbiota analysis of fermented milk samples with NGS
- Optimized protocol for viral metagenomics studies
- DNA extraction from frozen tumor samples
Related products
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Precellys® Evolution - Tissue Homogenizer
Bertin TechnologiesThis product has been discontinued. See the newer version of this product linked below.
Minilys - Personal Tissue Homogenizer
Bertin TechnologiesFlexible homogenizer for rapid sample disruption
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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Homogenizers and BlendersHomogenizers and blenders are used to homogenize various types of material, including tissue, cells, plants, food, soil, biological and chemical samples for subsequent analysis. Lab equipment for sample homogenization includes handheld and benchtop homogenizers, bead mill, ultrasonic and multiple sample homogenizers, high shear homogenizers and blenders. Homogenizer bead and probe multipacks are available to avoid cross contamination of samples, as well as cooling systems for homogenizing temperature-sensitive samples. Homogenizers are often used in the first step of extraction of DNA, RNA and proteins from tissue and cell samples. Find the best homogenizers and blenders in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Sample HomogenizerNGSTumorsTumor research focuses on understanding abnormal cell growth that leads to cancer. Identifying biomarkers, studying tumor microenvironments, and developing targeted therapies are critical for advancing cancer treatment. Early detection and personalized treatment options are key to improving outcomes for patients. Browse our peer-reviewed product directory to explore tools for tumor research, diagnostics, and cancer therapies; compare products, read customer reviews, and get pricing directly from manufacturers.homogenization