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NGS analysis of hereditary cancer predispositions at the Laboratory of Molecular Genetics, Jihlava Hospital

Agilent Technologies
1 Jul 2025

Explore how the Laboratory of Molecular Genetics at Jihlava Hospital, Czech Republic is utilizing the Agilent 4150 TapeStation system to streamline its NGS workflow, ensuring that only high-quality samples are sequenced.

The team is working to validate an NGS method designed to analyze hereditary predispositions to various cancers, including breast and colon. Their workflow involves isolating DNA from blood and buccal swab samples, with DNA concentration measured post-isolation with a fluorometer. Discover how the lab uses the TapeStation system at two stages, during library prep and before sequencing to assess the quality and quantity of their libraries prior to pooling.

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4150 TapeStation system

Agilent Technologies

The 4150 TapeStation system is an economic, entry-level system which comes with data processing software, reagents, and ScreenTape devices for analysis of sample size, quantity, and integrity of 1 to 16 samples. Delivering highly accurate and precise analytical evaluation, the systems fit perfectly into next-generation sequencing (NGS) or biobank workflows for handling smaller numbers of samples.

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Capillary ElectrophoresisCapillary electrophoresis (CE) is used to separate molecules based on charge, size and hydrophobicity. Pressure, voltage or a vacuum is used to introduce the sample to fused silica capillaries. Fluorescence, UV/Vis, UV or diode array detectors are used to visualize components. Types of CE systems include capillary electrochromatography (CEC), capillary zone electrophoresis and capillary gel electrophoresis. Find the best capillary electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.cancer genetics

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NGS analysis of hereditary cancer predispositions at the Laboratory of Molecular Genetics, Jihlava Hospital