Next-generation sequencing sample preparation with the NanoDrop One spectrophotometer

In this application note, Thermo Fisher Scientific demonstrates the accuracy of the NanoDrop^™ One/One^C spectrophotometer in quantifying and qualifying DNA isolated from saliva for downstream next-generation sequencing (NGS). NGS technology provides a genomic analysis to associate diseases such as cancer, heart disease, and diabetes with nucleic acid sequence aberrations. NGS can detect translocations, copy number variants (CNVs), insertions and deletions (indels), and single nucleotide variants (SNVs) with high sensitivity, all of which play a role in disease development. Saliva is a common sample type for genomics research as it provides a painless, inexpensive collection procedure.