ResourceLife Sciences
Next-Generation Amplicon-Based Deep Sequencing and Its Application to Characterize Hematological Malignancies
16 May 2013Recently, next-generation sequencing (NGS) platforms have evolved to provide an accurate and comprehensive means for the detection of molecular mutations. In this application note an amplicon-based deep sequencing strategy was used to demonstrate the power of deep sequencing for the molecular characterization of hematological malignancies. 95 cases were analyzed by applying next-generation sequencing from 454 Life Sciences, a Roche Company, to investigate 7 candidate genes.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LeukemiaKRASGenetic Variation