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New alternatives for low- and high-throughput RNA isolation/extraction promises simplicity, cost savings, and availability

Pall Life Sciences Products - Biopharmaceutical Division
19 Oct 2021

The new emphasis on research into viral disease therapies and test kits has placed greater importance on RNA isolation and extraction. In this application note, Pall highlights the new technologies available that promise greater simplicity and lower cost.

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Macrosep® Advance Centrifugal Devices

Pall Life Sciences - Laboratory, Food, Beverage

Quickly concentrate up to 20 mL of biological sample Pall Life Sciences Macrosep Advance Centrifugal Devices Features of the Microsep Advance Centrifugal Devices:  •  Rapidly concentrates 20 mL sample volumes to 0.5 mL.   •  Provides high recoveries, typically > 90%.   •  Low protein-binding Omega membrane and polypropylene housing minimize losses due to non-specific binding.   •  Versatile Omega membrane is available in a variety of MWCOs.   •  Built-in deadstop prevents spinning to dryness.   •  Color-coded for easy identificationApplications of the Microsep Advance Centrifugal Devices: Ultrafiltration   •  Concentrate and desalt proteins.   •  Exchange buffer or remove salt of chromatography eluates and gradient fractions.  •  Recover proteins or other molecules from cell culture supernatants.  Microfiltration   •  Remove particulate from aqueous solutions and clinical samples.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.PCRRNA ExtractionRT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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