Natural method for allergen identification using FastPrep-24TM 5G technology.

3 Dec 2019

In this application note, MP Biosciences discusses a fast method for protein extraction from pollen grains using western blot analysis.

FastPrep-24™ 5G

MP Biomedicals

The FastPrep-24™ 5G Instrument is quite simply the most advanced sample preparation system ever!   The only high-speed benchtop homogenizer featuring microprocessor-controlled, Hi-Def Touch Screen Programming makes the 5G the ultimate in ease, speed and performance for sample preparation applications where it is necessary to grind, lyse, or homogenize difficult and routine samples, allowing easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. Applications include but are not limited to: biological tissues, fluids and excretions cell cultures environmental sample other inorganic solid matrices. The FastPrep-24™ 5G Instrument uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Samples and buffers are added to Lysing Matrix Tubes containing specialized Lysing Matrix beads, supplied ready-to-use, certified nuclease-free and in a variety of sample type specific compositions. The sample tubes remain securely sealed during the processing and the single use design eliminates cross-contamination. Program parameters are easily set using the touch screen user interface, or choose from many Recommended Programs stored on the 5G's onboard computer. With a simple touch of the "run" button, in less than a minute your samples are completely lysed, super fast, every time! The highest power settings available ensure that DNA, RNA and proteins, even from difficult samples, are quickly liberated into the protective buffer, thus preserving their biological function. FastPrep-24™ 5G Exclusive Features Powerful - Highest speed range available, ideal for a wide range of applications and protects your sample Optimized - Cycle and pause programmable for true walk-away operation Convenient - >70 Recommended Programs for easy optimizations Intuitive - Interactive user-friendly interface and touchscreen Flexible - Optional sample holders allow processing from 2 - 50 mL tubes, under ambient or cryogenic conditions Smart - Integrated USB Port allows for software updates to be installed, and run history data to be downloaded to a laptop Safe - Electromechanical dome latch automatically secures processing chamber, with a simple finger touch Complete - Lysing Matrix Tubes and Purification Kits from One Source

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Allergen
Natural method for allergen identification using FastPrep-24TM 5G technology.