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Nanopore genomic DNA by Ligation

INTEGRA Biosciences
30 Apr 2024

The MIRO CANVAS NGS prep system protocol for Nanopore Genomic DNA by Ligation aims to generate research-only libraries for sequencing on the Oxford Nanopore Platform. The protocol details required materials, equipment, and consumables, emphasizing proper handling and storage. INTEGRA Biosciences outlines step-by-step procedures for preparing reagents, loading the MIRO Cartridge, and starting the run, with specific instructions to ensure accurate and efficient execution. Additionally, INTEGRA provides guidance on recovering the sample, finishing the run, and quantifying the final library product, along with a FAQ section addressing common queries and issues encountered during the process.

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MIRO CANVAS

INTEGRA Biosciences

MIRO CANVAS is a compact digital microfluidics platform for fully automated next generation sequencing (NGS) sample preparation. The revolutionary system drastically simplifies NGS workflows for more walk-away time and higher lab productivity, accelerating genomics discoveries.

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Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automation Supplies / AccessoriesAutomated accessories and consumables make lab processes fast, efficient and reliable. Useful automation accessories include microplate sealers, label printers and reagent reservoirs.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.

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Nanopore genomic DNA by Ligation