ResourceLife Sciences
MycoAlert Mycoplasma Detection Assays on Molecular Devices Microplate Readers
24 Aug 2015Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Mycoplasma cannot be detected by simply examining cell cultures under a microscope, a sensitive and reliable assay is needed to determine whether contamination is present. The MycoAlert™ Assay and MycoAlert PLUS Assay from Lonza provide a rapid and convenient way to detect viable mycoplasma in cell cultures using a luminescence microplate reader. This application note demonstrates how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert assays.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Contamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.MycoplasmaCell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.