Multiplexing LI-COR IRDye 680LT and IRDye 800CW

27 Feb 2019

This application note outlines a method for fluorescence-based detection of proteins on Western blots. Using fluorescence-based fluorophores increases sample throughput and can make multiplexing several fluorophores less time consuming.

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G:BOX Chemi XX9
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G:BOX Chemi XX9 gel imaging system has a high resolution camera for imaging multiple sample types and sizes, from fluorescence 1D to 2D gels to chemiluminescent blots. Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot.
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G:BOX Chemi XX6
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G:BOX Chemi XX6 gel imaging system has a high resolution camera for imaging multiple sample types and sizes, from fluorescence 1D to 2D gels to chemiluminescent blots. Your lab’s imaging system shouldn’t control how you detect proteins on Western blots. Chemiluminescence is great if you want sensitive detection of picogram or femtogram amounts, while fluorescence lets you quantify and detect multiple different proteins on one blot.
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G:BOX Chemi XRQ
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G:BOX Chemi XRQ is a cost-efficient chemiluminescence imaging and gel documentation system. For a laboratory that needs hassle-free chemiluminescent detection, as well as routine gel documentation, using the G:BOX Chemi XRQ’s powerful GeneSys software to switch between applications is simplicity itself.
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G:BOX Chemi XT4
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For high performance imaging, the new generation of G:BOX Chemi gel doc systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it. The G:BOX Chemi series features cameras with resolutions of 4m, 6m or 9m pixels. All have cooling which reduces the small amount of electronically generated noise to produce outstanding images with minimal background interference. These new generation cameras also have higher quantum efficiencies (QE) than most, helping the system to achieve outstanding levels of sensitivity. Whether using the system for standard fluorescence applications such as DNA, or for chemiluminescence imaging, the G:BOX Chemi will give you high quality results. In addition, by using the optional LED modules users can work with a unique automated multiplexed function capable of using up to 5 channels. Other LED modules can be used to turn the G:BOX Chemi into a highly sensitive IR capture system. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels. The stylish design with modular construction includes motor-driven lenses, filter wheels with the option of including a range of lighting choices for both Epi and transillumination applications. Syngene has just introduced the new high intensity HI-LEDs option for the G:BOX range, resulting in superior imaging performance for your fluorescence applications. Check the status of an image capture and access your recently captured images from any computer, smartphone or tablet on the same network as G:BOX Chemi XT4 gel doc system using the new "Status Link" feature. This is ideal for any busy laboratory. The system comes complete with unlimited copies of GeneTools analysis software.
(1)

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Western BlottingFluorescence

G:BOX Chemi XT4

Syngene

For high performance imaging, the new generation of G:BOX Chemi gel doc systems provide the user with an extended range of applications covering fluorescence and chemiluminescence. All these systems now utilise Syngene’s groundbreaking GeneSys automatic control software. Driven from a database containing hundreds of capture protocols, full manual control is available should you wish to use it. The G:BOX Chemi series features cameras with resolutions of 4m, 6m or 9m pixels. All have cooling which reduces the small amount of electronically generated noise to produce outstanding images with minimal background interference. These new generation cameras also have higher quantum efficiencies (QE) than most, helping the system to achieve outstanding levels of sensitivity. Whether using the system for standard fluorescence applications such as DNA, or for chemiluminescence imaging, the G:BOX Chemi will give you high quality results. In addition, by using the optional LED modules users can work with a unique automated multiplexed function capable of using up to 5 channels. Other LED modules can be used to turn the G:BOX Chemi into a highly sensitive IR capture system. An edge lighting option can also be used for 2D gel capture including the use of DIGE gels. The stylish design with modular construction includes motor-driven lenses, filter wheels with the option of including a range of lighting choices for both Epi and transillumination applications. Syngene has just introduced the new high intensity HI-LEDs option for the G:BOX range, resulting in superior imaging performance for your fluorescence applications. Check the status of an image capture and access your recently captured images from any computer, smartphone or tablet on the same network as G:BOX Chemi XT4 gel doc system using the new "Status Link" feature. This is ideal for any busy laboratory. The system comes complete with unlimited copies of GeneTools analysis software.

(1)

Multiplexing LI-COR IRDye 680LT and IRDye 800CW

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G:BOX Chemi XX9

G:BOX Chemi XX6

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G:BOX Chemi XT4

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