Multiplexed assessment of mitochondrial function combining the Agilent Mito-rOCR assay with live-cell imaging

The Agilent Mito-rOCR assay is an innovative fluorescence plate reader-based solution for measuring relative mitochondrial respiration rates. Its unique design, compatible with multimode plate readers, allows for the simultaneous analysis of mitochondrial function and other cellular processes. The multiplexed application of the Mito-rOCR assay enables the extraction of multiple parameters from a single sample, offering a more comprehensive understanding of cellular metabolism and function.

This application note outlines the basic workflow of the multiplexed Mito-rOCR assay with other plate reader-based assays and presents two example applications that combine Mito-rOCR measurements with mitochondrial imaging for deeper characterization of mitochondrial function.