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Multiplex Detection of Stem Cell Pluripotency Markers in Reference Cancer Cell Lines

Merck
21 Jun 2013

Several proteins, including Oct 3/4, Sox2, KLF4 and Nanog, play a critical role in the maintenance of pluripotency in stem cells. Overexpression of one or a combination of these factors has been reported in solid tumors from a variety of tissues. The presence of pseudogenes and differentially expressed protein isoforms complicates the accurate detection of the physiologically relevant forms of these proteins in cancer stem cell research. In this poster read about the development of MILLIPLEX® MAP multiplex assays for the simultaneous, specific and sensitive detection of the pluripotency markers Oct 3/4, Sox2, KLF4 and Nanog as well as several other stem cell-relevant proteins in cellular lysates.

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MILLIPLEX MAP Human Stem Cell Pluripotency Magnetic Bead Panel 1 - Stem Cell Multiplex Assay

Merck

Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Panel 1 (Cat. No. 48-617MAG) is a premixed panel to be used for the simultaneous quantification of the following 4 analytes in cell lysates: c-Myc, Nanog, Oct 3/4, and Sox2. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay. Background Information: The expression of the transcription factors Oct 3/4, Sox2 and Nanog play a critical role in the maintenance of pluripotency and self renewal in stem cells. c-Myc, a member of the helix-loop-helix-leucine zipper family, is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. In stem cells it seems to control the balance between stem cell self-renewal and differentiation. Thus the presence of the four proteins Oct-3/4, Sox2, Nanog and c-Myc are indicators for the pluripotency of iPS cells and human embryonic stem cells. Applications: This MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Panel 1 is a Stem Cell Characterization multiplex assay. The analytes available for this multiplex kit are: c-Myc, Nanog, Oct 3/4, Sox2. 

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MILLIPLEX MAP Human Stem Cell Pluripotency Magnetic Bead Panel 2 - Stem Cell Multiplex Assay

Merck

Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Kit 2 (Cat. No. 48-620MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates: E-Cadherin, EpCam, KLF4, LIN-28, and the glycostructures TRA-1-60, TRA-1-81 and SSEA1. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay. Background Information: The expression of transcription factors, translational regulators and trans-membrane proteins, as well as cell surface glycostructures, play critical roles in the maintenance of pluripotency and self renewal in stem cells and can serve as markers of cell status. Applications: This MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Panel 2 is a Stem Cell Characterization multiplex assay. The analytes available for this multiplex kit are: E-Cadherin, EpCam, KLF4, LIN-28, SSEA1, TRA-1-60, TRA-1-81. 

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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Multiplex Detection of Stem Cell Pluripotency Markers in Reference Cancer Cell Lines