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Multiple Detector Approaches to Protein Aggregation by SEC

6 Aug 2020

Methods are given for the use of Agilent Bio-SEC columns, with LS, UV and RI detection, for quantification or quality control of protein aggregates

1260 Infinity II Multidetector GPC/SEC System

Agilent Technologies

With the 1260 Infinity II Multidetector GPC/SEC System, you can select from any combination of detection by refractive index, light scattering, and viscometry. The system delivers accurate, absolute molecular weights and sizes, and can be used to analyze a wide range of polymers, regardless of molecular weight range or solvent. Each detector has independent temperature control to ensure that high levels of precision and reproducibility can easily be achieved from one day to the next.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Multiple Detector Approaches to Protein Aggregation by SEC