ResourceLife Sciences
mRNA poly A tail degradation detection on a Biozen oligo column
25 May 2023The poly A tail of a messenger RNA (mRNA) is a repeat sequence of adenosine (A) that is an emergingly popular modality among vaccine and gene therapy developers. Grown at the 3’ end, the increasing length of this tail provides additional stability to the “core” sequence that invokes the generation of a protein and subsequent immune response from the host. In this application note from Phenomenex, explore how to distinguish a naturally degraded poly A tail with single nucleotide resolution through Liquid Chromatography (LC) and High-Resolution Mass Spectrometry (HRMS).
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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.