Morphologic and Location-Based Classification of Differentiating Erythroid Lineage Cells using the ImageStream

30 Nov 2010

In this application note human CD34-positive early hematopoietic cells were cultured to promote differentiation into erythroid lineage cells. During the differentiation program cells were sampled at 10, 13, or 16 days followed by staining with a nuclear dye and for expression of CD71 and glycophorin A, then analyzed immunophenotypically and morphologically on the ImageStream.

Image Stream®X Mark II Imaging Flow Cytometer

Merck

The revolutionary ImageStream®X Mark II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone. This instrument produces multiple high-resolution images of every cell directly in flow, including brightfield and darkfield (SSC), and up to 10 fluorescent markers with sensitivity exceeding conventional flow cytometers. Compared to its predecessor, the new ImageStream®X Mark II Imaging Flow Cytometer offers a streamlined workflow, greater flexibility, and optimizations for rare cell applications. Taken together, the capabilities of the ImageStream®X Mark II make it superior for traditional flow applications while greatly expanding the scope of flow cytometry. Applications include the study of cell-cell interactions, phagocytosis, apoptosis and autophagy, the characterization of circulating tumor cells, and many others.

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Morphologic and Location-Based Classification of Differentiating Erythroid Lineage Cells using the ImageStream