ResourceLife Sciences
Monitoring of Insulin Granule Packaging in Live Cells using HomoFRET-FP Detection
20 Jul 2017This application note describes a high throughput screening compatible cell based assay that uses a preproinsulin-mCherry (PPI-mCherry) system. This assay can be used to identify compounds that modify insulin granule packing and is a novel method for the assessment of protein-protein interactions in live cells. The application exploits the fact that when a fluorophore is at a high local concentration FRET can occur between the same type of fluorophores.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.DiabetesDiabetes is a metabolic disorder characterized by high blood sugar levels, either due to insufficient insulin production or resistance to its effects. Ongoing diabetes innovation focuses on developing better diagnostic tools, treatments, and preventive measures. Browse our peer-reviewed product directory to find the best diabetes diagnostic tools, compare products, check reviews, and get pricing directly from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.