Monitor NF-κB activation with a sensitive dual reporter assay on the SpectraMax iD5 microplate reader
24 Feb 2021Reporter genes are valuable tools for studying gene expression, serving as surrogates for genes involved in various signaling pathways and disease conditions, with Luciferases being the most common of these.
In this application note, Molecular Devices demonstrates how the DuoLuc reporter assay and SpectraMax iD5 reader can be utilized to detect nuclear factor-κB (NF-κB) activation in mammalian cell models.
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SoftMax® Pro Microplate Data Acquisition & Analysis Software
Molecular Devices®SoftMax ® Pro Software is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step has been optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. FDA 21 CFR Part 11 compliance tools are available for regulated laboratories providing end-to-end chain of custody.
SpectraMax DuoLuc Reporter Assay Kit
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