Monitor E. coli cultures with the BioLector and Multisizer 4e

Escherichia coli (E. coli) is a facultative anaerobic bacterium, easily cultured in labs, making it a key model organism in genetics, microbiology, and biotechnology. It is commonly used for large-scale production of therapeutic proteins, with 30% of approved therapeutic proteins produced using E. coli. Beckman Coulter Life Sciences demonstrates the use of the BioLector microbioreactor and the Multisizer 4e Coulter Counter to optimize E. coli culture conditions and characterize cell growth. The BioLector is small-scale, automated system that enables high-throughput screening, cultivation parameter monitoring and feeding strategy optimization. The Multisizer 4e is an accurate and flexible particle characterization device with a sizing range of 0.2–1600 μm. Generated data are processed using patented digital pulse processing technology and can be saved and later re-analyzed.