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Molecular Characterization and Quantitation of Lipids with High Resolution Accurate Mass Tandem MS Techniques

SCIEX
15 Jun 2017

This application note reviews a data independent Infusion MS/MSALL Workflow, and it's application towards the comprehensive analysis of biological lipid extracts. Quadrupole time of flight (QqTOF) instruments are well suited to data-independent experiments as they provide high resolution and mass accuracy and without speed or mass range limitations. Furthermore, the strength of QqTOF instruments for lipid molecular identification applications has been well demonstrated.

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MarkerView software does the heavy lifting for you to rigorously mine data and provide statistical analysis and visualization of large numbers of samples. MarkerView software is ideal for profiling and comparing the composition within your samples in order to gain valuable insight into any trends within your mass spectral. This post-acquisition analysis software reduces the need for manual intervention empowering you to explore statistical correlations with direct connections back to the raw data from your SCIEX mass spectrometer, so you can confidently and effectively solve even your most complex analysis.  

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Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.MetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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