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miRNA Profiling from Blood – Challenges and Recommendations

QIAGEN
18 May 2015

The discovery of stable miRNA species circulating in blood has led to increased research focus on disease-related variations in serum and plasma miRNA expression and the possibility that such variations could serve as noninvasive biomarkers for disease. Working with serum and plasma miRNA presents various challenges in purification and characterization. This paper outlines QIAGEN® recommendations for robust purification and quantification, as well as reliable data normalization and analysis.

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miScript miRNA PCR Arrays

QIAGEN

The miScript miRNA PCR Arrays accurately analyze the expression of multiple microRNA (miRNA) sequences at the same time on ANY real-time PCR instrument. This new generation of miRNA PCR Array builds on the original strengths of the RT² miRNA PCR Arrays* with new pathway content, expanded assay coverage plus added species all with the flexibility and sample preparation advantages of the miScript PCR System. The available 96- or 384-well plates or Rotor Disc 100 arrays contain SYBR Green optimized and experimentally validated assays for either entire human, mouse, rat and dog miRNA genomes (miRNomes) or pathway-focused sets of miRNA assays. Our proprietary miRNA detection technologies enable uniformly high PCR amplification efficiencies, allowing simultaneous detection of miRNA under uniform cycling conditions. These technologies also provide the miScript miRNA PCR Array with high sensitivity, specificity, and accuracy. miScript miRNA PCR Arrays are currently available for the human, mouse, rat and dog species, and individual miRNAs can be analyzed independently with miScript Primer Assays.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.Blood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.miRNA

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miRNA Profiling from Blood – Challenges and Recommendations