MiniPrep PhyTip Columns for plasmid DNA purification

15 Mar 2022

In this application note, discover how the MiniPrep PhyTip® Columns for plasmid DNA purification provide an alternative, fully automated, high throughput solution for performing rapid DNA minipreps.

Biotage Miniprep PhyTip Columns for Plasmid DNA Purification

Biotage

Lysate Direct PhyTip® Column Technology consists of novel purification columns that are composed of a pipet tip containing plasmid DNA binding resin and a thin, inert frit screen at the end of the tip to retain the resin. The unique design of the column allows for DNA binding directly from cell lysates in the presence of precipitates and debris without sample clearing. By eliminating the need for centrifugation or filtration, lysate direct PhyTip columns for plasmid DNA purification enable utilization of liquid handing automation found in high throughput laboratories to easily purify up to 96 samples in parallel. In addition, lysate direct PhyTip® columns are also compatible with the MEA Personal Purification System available from PhyNexus for 12-at-a-time sample processing. Features include: No filtration or centrifugation of lysate required Consistent purification of up to 15 μg Transfection grade and sequencing-ready 96 samples at a time Suitable for transfection, transformation, sequencing, PCR and cloning Fast and fully automated from the cell pellet, no lysate centrifugation required

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.
MiniPrep PhyTip Columns for plasmid DNA purification