ResourceLife Sciences
Minimize Loss of Transfected Cells During Luminescence Assays
5 Dec 2018Cell attachment is considered to be critical for many cell culture applications since it triggers for instance differentiation, metabolic activity and gene expression. Medium changes and washing steps especially in automated culture processing or high content screenings (HCS) can cause remarkable cell loss.. Here the BRANDplates® cellGrade™ plus surface were tested against comparable TC-treated microplates from different well known manufacturers regarding their ability to support cell proliferation and attachment after transfection. In this experiment success of transfection and cell retention during washing steps was followed by measuring luminescence signal intensity generated by luciferase transfected cells.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.Cell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.LuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.