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Miniaturized GloSensor cAMP Assay by Promega, Using the Echo® Liquid Handler

Promega Corp.
26 Jul 2015

As biological assays used for HTS and uHTS are miniaturized, it is critical to maintain assay sensitivity while minimizing reagent volumes and maximizing throughput. The GloSensor cAMP Assay provides an extremely sensitive and easy to use, real-time luminescent assay format for the interrogation of over-expressed or endogenous GPCRs that signal via changes in the intracellular concentration of cAMP. This scientific poster demonstrates optimization of the GloSensor cAMP Assay in low-volume format using a stably transfected HEK293 cell line model expressing the melanocortin 4 receptor (MC4R) and a cAMP-sensing variant of firefly luciferase.

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Dual-Glo® Luciferase Assay System

Promega Corp.

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false positive and false negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.

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Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.LuciferaseAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.hiPS Cell Lines

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