Miniaturization of an HTRF Methyltransferase Assay that Detects Histone Modifying Activity

The most sensitive microplate reader for high-throughput screening

Imagine preparing assay plates for siRNA screening, compound screening with biochemical and cell-based assays, PCR reactions and more all on a single liquid handling platform, with no tips and no cross-contamination. Labcyte has developed new acoustic droplet ejection (ADE) parameters that deliver built-in intelligence to enable users to transfer many different fluid types from a single instrument. All Echo systems come pre-calibrated so that you can switch among different fluid types with just a click of the mouse. No further adjustment is required for you to be productive immediately. These developments combine the latest discoveries in wavelength, power and timing requirements for ADE with real-time fluid measurement at multiple points during a run. The resulting algorithms solve some of the most significant liquid handling challenges with volatile, low-surface tension and/or meniscus-shifting fluids. Volumetric accuracy and precision for all fluids tested so far—including buffers, nucleic acid and protein solutions, surfactants, serum and cell culture media—are comparable to established performance with DMSO. The Echo® 550 liquid handler introduced the world to acoustic droplet ejection (ADE). This award-winning instrument remains the proven choice for low-volume liquid handling in leading pharmaceutical and biotechnology companies worldwide. Like the Echo 520 and Echo 555 liquid handlers, the Echo 550 uses "touchless" ADE transfer technology. ADE uses sound energy to transfer 2.5 nL droplets from Echo-qualified 384- and 1536-well source plates into 96-, 384-, 1536- and 3456-well destination plates. This unique touchless technology eliminates consumable tip costs, washing, and cross-contamination. Unsurpassed volumetric precision (≤ 8% CV) and accuracy (≤ 10% CV) ensure superior results. Independent studies have shown that Echo liquid handlers eliminate false negatives due to sample loss on tip surfaces in low-concentration transfers. Like the Echo 555 liquid handler, the Echo 550 liquid handler reports both fluid volume and DMSO hydration. Applications: Compound management Compound screening siRNA screening Biochemical assays Cell-based assays PCR Genotyping Gene expression