Mimicking Real Tissue Using 3D Cell Cultures Allows Cells to Grow and Propagate Better

13 Feb 2013

Technological advances over the past couple of years have enabled 3D scaffolds to be engineered that create an in vivo proxy that is more realistically mimics living cellular environments. This application note demonstrates that the Freedom EVO workstation can simply and routinely process cell cultures in Alvetex Scaffold in a 96-well plate format. The results show that when combined these systems can provide cell biologists with higher throughput as well as higher level of standardization for both basic research and drug discovery.

Freedom EVO® with Dual Liquid Handling Arms

Tecan

Tecan’s Freedom EVO series of automated workstations offer unrivalled processing speed and flexibility thanks to a unique feature that allows two liquid handling (LiHa) arms to act completely independently. Each arm of eight variable-spacing pipetting tips can be adapted to handle high volumes, for example of cell culture media, as well as low volumes of precious reagents or samples. The dual LiHa arms can run in parallel with the robotic manipulator (RoMa) arm which moves labware around the worktable, and are fully compatible with Tecan’s extensive range of application options, including storage elements, stirrers and incubation, separation or reading devices. This capability is a great asset for a variety of applications, including hit picking, serial dilutions, consecutive sample transfer and other aspects of assay preparation, and can greatly enhance the liquid handling power of any laboratory.

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Tecan Freedom EVO® Workstation

Tecan

The Freedom EVO platform offers fully automated liquid handling and robotics for life sciences and diagnostics. This flexible system offers virtually unlimited options, which can be tailored to provide individual solutions.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.
Mimicking Real Tissue Using 3D Cell Cultures Allows Cells to Grow and Propagate Better